Ten of Australia's major freight companies have entered into agreements with WesternSydneyAirport.
This sees the airport work with the air-freight industry to design concepts for an on-airport freight precinct and begin discussions about potential operations.
Airport chief executive GrahamMillett said the airport has the potential to become Sydney's most important freight hub, generating many of the airport's thousands of jobs.
"The unrestricted 24/7 operations will be key to unlocking new export opportunities for businesses and producers," he said. "Goods produced in NSW will no longer have to be trucked to Brisbane or Melbourne to reach lucrative Asian markets overnight."
Freight companies signing up are AustraliaPost (including StarTrack), DBSchenker, DHLExpress, DSVAir&Sea, FedEx, MenziesAviation, Swissport, QantasFreight, SkyroadLogistics and Wymap.
Mr Millett said insights from these international freight leaders would help the airport optimise design and functionality.
"The growth of online shopping and demand from Asia for Australian fresh produce has had an enormous influence on the international freight market."
The airport's first stage has potential to process 220,000 tonnes of air freight annually. The freight hub will have dedicated road access off The Northern Road, separating heavy vehicles from passenger traffic, which will enter the airport from the M12 on the site's opposite side.